Journal: Experimental gerontology

Article Title: Aerobic exercise modulates aortic chondrogenesis and calcification via 5-methoxytryptophan and P38MAPK in atherosclerotic rats.

doi: 10.1016/j.exger.2025.112722

Figure Lengend Snippet: Fig. 7. B–F indicates the effects of aerobic exercise on aortic P38MAPK, pp38MAPK, pp38MAPK/P38MAPK, collagen II, and IL-6 protein expression in AS rats, compared with Con group, * p < 0.05 and **p < 0.01; compared with AS group # p < 0.05 and ##p < 0.01.

Article Snippet: The PVDF transmembrane was transfected with the addition of primary antibodies pp38 MAPK (1:1000, ab4822, abcam), p38 MAPK (1:1000, NB100–56446, NOVUS), collagen II (1:1000, ab34712, abcam), IL-6 (1:1000, NBP2–89149, NOVUS), incubated for 12 h, secondary antibody incubated for 2 h, ECL developed, fixed, exposed and scanned to process the gray value of each band.

Techniques: Expressing

Journal: Molecular psychiatry

Article Title: n-3 polyunsaturated fatty acid deprivation in rats decreases frontal cortex BDNF via a p38 MAPK-dependent mechanism.

doi: 10.1038/sj.mp.4001888

Figure Lengend Snippet: Figure 3 Frontal cortex PKA (a), CaMK IV (b), PKC (c) and p44 MAPK (d) activities in n-3 PUFA þ and n-3 PUFA rats. Representative frontal cortex nuclear immunoblots of phosphorylated MAPK p42 and p44 protein levels in n-3 PUFA þ and n-3 PUFA rats (e). Data were compared using unpaired t-tests, mean7s.e.m., n = 10 independent samples per group, *P < 0.05.

Article Snippet: Nuclear extracts protein blots were incubated overnight with antiphosphorylated p42 or p44 MAPK (Santa Cruz) (1:200 dilution) or antiphosphorylated p38 MAPK antibody (R&D Systems, 1:200 dilution), followed by HRPconjugated secondary antibodies (Bio-Rad).

Techniques: Western Blot

Journal: Molecular psychiatry

Article Title: n-3 polyunsaturated fatty acid deprivation in rats decreases frontal cortex BDNF via a p38 MAPK-dependent mechanism.

doi: 10.1038/sj.mp.4001888

Figure Lengend Snippet: Figure 4 Frontal cortex cytosolic p38 MAPK activity in n-3 PUFA þ and n-3 PUFA rats (a). Phosphorylated p38 MAPK protein levels in n-3 PUFA þ and n-3 PUFA rats (b). Data were compared using unpaired t-tests, mean7 s.e.m., n = 10 independent samples per group, *P < 0.05.

Article Snippet: Nuclear extracts protein blots were incubated overnight with antiphosphorylated p42 or p44 MAPK (Santa Cruz) (1:200 dilution) or antiphosphorylated p38 MAPK antibody (R&D Systems, 1:200 dilution), followed by HRPconjugated secondary antibodies (Bio-Rad).

Techniques: Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Computational and In Vitro Analysis of Plumbagin’s Molecular Mechanism for the Treatment of Hepatocellular Carcinoma

doi: 10.3389/fphar.2021.594833

Figure Lengend Snippet: (A) Protein expression levels of LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK in SMMC-7721 cells measured by Western blot after treatment with 5 μM PL, 10 μM 3-MA autophagy inhibitor, 10 μM rapamycin autophagy agonist, 10 μM SB202190, and 10 μM SB203580 p38 MAPK inhibitor. (B) LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK were used as internal reference total proteins for gray value analysis. * p < 0.05, ** p < 0.01 *** p < 0.001, compared with the PL group. # p < 0.05, ## p < 0.01 ### p < 0.001 vs. the control group. (C) Protein expression levels of LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK in BEL-7404 cells measured by Western blot after treatment with 5 μM PL, 10 μM 3-MA autophagy inhibitor, 10 μM rapamycin autophagy agonist, 10 μM SB202190, and 10 μM SB203580 p38 MAPK inhibitor. (D) LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK were used as internal reference total proteins for gray value analysis. * p < 0.05, ** p < 0.01 *** p < 0.001, compared with the PL group. # p < 0.05, ## p < 0.01 ### p < 0.001 vs. the control group.

Article Snippet: Plumbagin (PL) was purchased from Sigma-Aldrich (St. Louis, MO, United States) with purity ≥98%; the Acridine Orange (AO)/Ethidium bromide (EB) Double Stain Kit was from Solable Technology (Beijing, China); N-acetyl-l-cysteine, SB203580, and SB202190 were from Sigma-Aldrich (St. Louis, MO, United States); SC-79, MEK2206, 3-MA, and Z-VAD-FMK were from Selleck (Texas, United States); the BCA Protein Assay Kit, ROS Assay Kit, Annexin V-FITC Apoptosis Detection Kit and Cell lysis buffer for Western were all obtained from Beyotime Biotechnology (Shanghai, China); antibodies against Akt, phospho-Akt, mTOR, phospho-mTOR, p38 MAPK, phospho-p38 MAPK, PI3K, phospho-PI3K, LC3B, cleave-RP, and cleave-caspase 3 were from Cell Signaling Technology, Inc. (Boston, MA, United States); and β-p38 MAPK was purchased from Boster Technology (Wuhan, China).

Techniques: Expressing, Western Blot, Control